DNA

Part:BBa_K4329003:Design

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-25)


Quorum sensing suicidal system-part1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter is an efficient promoter for

Lac operator inhibits protein and enzyme production without the presence of IPTG

LuxR is a common system used in the quorum system. It is used to detect AHL in the environment.

T7 terminator is used to stop the transcription.

QS promoter is the original sequences from Vibrio fischeri which controls the expression of Lux IABC gene expression.

Source

BBa_Ka3882000 from BS_United_China in 2021

References

T. 0. Baldwin, J. H. Devine, R. C. Heckel, J.-W. Lin, and G. S. Shadel Department of Biochemistry and Biophysics, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843, USA